Journal: Cell Reports Methods

Article Title: Long-term imaging of individual mRNA molecules in living cells

doi: 10.1016/j.crmeth.2022.100226

Figure Lengend Snippet: Design principles of SunRISER are generalizable to orthogonal stem loops and protein-tagging systems (A) HeLa cells transfected with detection plasmids phage-cmv-CFP-24×MS2 (top) and phage-cmv-CFP-24×MS2V6 (bottom) stem-loop variants of SunRISER with ubc-nls-MCP-5×SunTag show similar characteristics and intensity distributions, quantified in histograms (right, top: n = 30 for cell numbers and n = 8,227 for spots numbers; bottom: n = 21 for cell numbers and n = 4,627 for spots numbers). (B) HeLa cells transfected with detection plasmid phage-cmv-cfp-24×pp7 with cmv-sfGFP-GB1-Nb-gp41 and ubc-nls-PCP-12×MoonTag, quantified in histograms (right, n = 19 for cell numbers and n = 4,534 for spots numbers). We note that the MoonRISER example can be further optimized as it uses a longer 12× MoonTag and a nanobody that has different binding properties. Cells were imaged with 60× wide-field microscope 24 h after transfection and quantified with dNEMO ( Kowalczyk et al., 2021 ) Scale bar: 10 μm. See also Figure S5 .

Article Snippet: 24×MS2V6 stem-loops from pET259-pUC57-24×MS2V6 (Addgene #104391 ( );) was amplified using BamHI and SacII sites to label cfp the same way as other two stem-loops.

Techniques: Transfection, Plasmid Preparation, Binding Assay, Microscopy

Journal: Cell Reports Methods

Article Title: Long-term imaging of individual mRNA molecules in living cells

doi: 10.1016/j.crmeth.2022.100226

Figure Lengend Snippet:

Article Snippet: 24×MS2V6 stem-loops from pET259-pUC57-24×MS2V6 (Addgene #104391 ( );) was amplified using BamHI and SacII sites to label cfp the same way as other two stem-loops.

Techniques: Recombinant, Software

Journal: Cell metabolism

Article Title: Local Mitochondrial ATP Production Regulates Endothelial Fatty Acid Uptake and Transport.

doi: 10.1016/j.cmet.2020.05.018

Figure Lengend Snippet: (A) 72-hour knockdown of FATP3 and FATP4 with 25 nM siRNA reduced FA uptake 25–33% as compared to scrambled control siRNA (siC).

Article Snippet: LbNOX was obtained as an addgene plasmid (gift from Vamsi Mootha, addgene # 75285) while Human FATP4 cDNA was synthesized as an IDT gBlock.

Techniques: Knockdown, Control

Journal: Cell metabolism

Article Title: Local Mitochondrial ATP Production Regulates Endothelial Fatty Acid Uptake and Transport.

doi: 10.1016/j.cmet.2020.05.018

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: LbNOX was obtained as an addgene plasmid (gift from Vamsi Mootha, addgene # 75285) while Human FATP4 cDNA was synthesized as an IDT gBlock.

Techniques: Purification, Virus, Recombinant, Transfection, Infection, Plasmid Preparation, Drug discovery, Gel Extraction, Cloning, ATP Bioluminescent Assay, Mutagenesis, CyQUANT Assay, Proliferation Assay, Isolation, Negative Control, Sequencing, Synthesized, Software, Membrane